CHO 302 Serum-Free ADCF Powder Medium

CCM-SF-0011 is a dry powder serum-free medium for CHO cell culture applications where defined composition, animal-derived component-free classification, and scalable handling are required. The format is suitable for facilities that hydrate media in-house and qualify preparation procedures under established process controls. This configuration is supplied without phenol red, without L-glutamine, and without sodium bicarbonate, allowing users to add these components according to internal specifications, feed strategy, and bioreactor operating conditions.

Key release specifications include pH 6.4–7.0, osmolality 290–330 mOsmol/kg, and endotoxin not more than 3 EU/mL. The recommended culture environment is 37 °C with 5–10% CO2 after complete preparation and adjustment according to the user’s validated protocol. The medium is supplied as a 100 L powder pack and is categorized within serum-free media for CHO-based research, development, and manufacturing support workflows.

• Serum-free suspension or adapted CHO culture using CHO K1, CHO-S, CHO-GS, and CHO DG44 host systems.
• Animal-derived component-free media preparation for recombinant protein expression, cell expansion, and upstream process development.
• Scale-up studies where powder media logistics, batch-to-batch documentation, and controlled hydration procedures are preferred over ready-to-use liquid formats.
• Media qualification programs that require user-controlled addition of L-glutamine and sodium bicarbonate based on target pH, osmolality, and CO2 settings.
• Procurement programs for research, pilot, and production support teams that need defined serum-free medium with clear specifications and lot traceability.

Store unopened powder at 2–8 °C protected from light. The stated shelf life is 24 months when stored under recommended conditions and handled with appropriate material controls. This product ships on blue ice to support temperature-managed transit. During preparation, use qualified water, calibrated mixing equipment, and established filtration and pH-adjustment procedures. Because phenol red, L-glutamine, and sodium bicarbonate are not included, verify all additions, final osmolality, and final pH before release to cell culture use. Avoid repeated warming of unopened material, minimize exposure to ambient humidity, and keep container closures secure after sampling in accordance with site procedures.

CellCultureMedia supplies CCM-SF-0011 with specification-focused documentation for procurement and technical review. Manufacturing and quality controls are ISO-aligned, with lot-specific documentation available to support incoming material qualification. A Certificate of Analysis can be requested through our quality documentation channel, and procurement teams may use request a quote for bulk pricing, forecasted orders, or scheduled deliveries. Free worldwide shipping is available, with temperature-managed packing selected for this powder medium. For regulated internal environments, users should qualify the medium in their own cell line, vessel format, preparation procedure, and acceptance criteria before routine use.