Animal-Origin-Free Serum-Free CHO Medium

This 100 mL serum-free CHO medium is specified for animal-origin-free culture systems where reduced undefined inputs, consistent lot qualification, and clear release criteria are required. It is suitable for CHO K1, DG44-derived, GS-selected, and suspension-adapted CHO workflows used in recombinant protein expression, antibody production research, vaccine process development, and upstream biopharmaceutical manufacturing studies. The medium is supplied as a sterile-filtered liquid and is part of the serum-free media portfolio from CellCultureMedia.

Key specifications include pH 7.0–7.4, osmolality 270–340 mOsmol/kg, endotoxin not more than 3 EU/mL, and a 12-month shelf life when stored as recommended. The formulation excludes phenol red to support workflows where optical interference or pH indicator color is not desired. L-glutamine and HT supplement are not included, allowing process engineers to control supplementation strategy according to cell line requirements, feed design, and internal validation protocols.

• Serum-free expansion and maintenance of CHO production cultures under 37 °C, 5–10% CO2 incubation conditions.
• Small-scale screening, shake flask studies, seed train evaluation, and process development requiring animal-origin-free medium chemistry.
• Recombinant protein and antibody expression research where serum-free inputs support cleaner downstream development and more consistent analytical baselines.
• Biopharmaceutical and vaccine manufacturing development programs requiring defined medium supply, endotoxin control, and lot-level documentation.
• Phenol-red-free experimental designs involving optical density, fluorescence, imaging, or other readouts where color indicators may affect measurement.
• Controlled supplementation studies in which users add L-glutamine, stable glutamine alternatives, HT components, feeds, or productivity enhancers according to internal protocols.

Store CCM-SF-0012 at 2–8 °C protected from light. Do not expose the bottle to repeated warming cycles, direct light, or uncontrolled room-temperature holding. Mix gently before use and apply aseptic technique when opening, aliquoting, or supplementing. The product ships on blue ice to maintain refrigerated transit conditions, and users should place material into qualified cold storage immediately upon receipt. For best planning, align receipt dates with cell bank recovery, seed train initiation, or media qualification runs so the 12-month shelf life is used efficiently within internal inventory systems.

CellCultureMedia supplies CCM-SF-0012 for research and bioprocessing use with ISO-aligned manufacturing controls, sterile filtration, defined release specifications, and lot traceability. A Certificate of Analysis is available on request and can support incoming quality review, supplier qualification, and process documentation. Procurement teams can use the quality documentation page to review general quality practices or submit an inquiry through request a quote for lead time, volume pricing, and shipping confirmation. Free worldwide shipping is available for eligible orders, including this 100 mL configuration priced at $319.44.