Animal-Origin-Free Serum-Free 293 Medium

CCM-SF-0057 is a ready-to-use liquid medium for 293F-type and other HEK293-derived suspension cell culture systems used in recombinant protein expression, transient transfection process development, and upstream biomanufacturing research. The formulation is serum-free and animal-origin-free, reducing reliance on undefined biological inputs while supporting consistent handling in controlled laboratory and pilot-scale environments. For related formulations, buyers can review the serum-free media category or consolidate requirements through a single procurement request.

Key supplied specifications include pH 7.0-7.4 and osmolality 270-340 mOsmol/kg. The medium is filter sterilized, with an endotoxin specification of ≤3 EU/mL and a stated shelf life of 12 months when stored as directed. The formulation contains D-glucose, 2 mM L-glutamine, and 2 mM L-alanyl-glutamine; phenol red is not included. The product is intended for culture at 37 °C in a humidified incubator with 5-10% CO2, according to the receiving laboratory's established cell line adaptation and passage procedures.

• Serum-free suspension culture of HEK293-derived cells, including 293F-type systems used in shake flasks, spinner vessels, and controlled bench-scale bioreactors.
• Transient transfection workflows for plasmid-based expression studies, process parameter screening, and small-batch production of recombinant proteins or biologic research materials.
• Animal-origin-free process development where laboratories need to minimize undefined serum components and align upstream media selection with later-stage scale-up requirements.
• Comparability studies for media screening, feeding strategy evaluation, inoculum expansion, and productivity optimization in vaccine research and biopharmaceutical development settings.
• Phenol-red-free culture conditions where optical readouts, color-sensitive assays, or analytical sampling programs benefit from reduced background interference.

Store CCM-SF-0057 at 2-8 °C protected from light. The product ships on blue ice and should be transferred to refrigerated storage promptly after receipt. Do not use beyond the assigned expiry date, and avoid prolonged exposure to ambient temperature during routine sampling or media changes. As with all serum-free media, aseptic technique is required after opening; dispense only in a qualified biosafety cabinet or equivalent controlled environment. For best operational control, record lot number, opening date, storage temperature, and any in-house hold-time limits in the batch or laboratory record.

CellCultureMedia supplies CCM-SF-0057 for research and bioprocessing use with specification-based quality controls aligned with ISO-style manufacturing and release practices. Lot-specific documentation, including a Certificate of Analysis, is available on request through the quality documentation page. Standard orders include free worldwide shipping where available, and the team can support recurring, bulk, or multi-site purchasing through the request quote workflow. Procurement teams may reference CCM-SF-0057, 500 mL format, when requesting availability, lead time, and bulk pricing.